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WET (Whole Effluent Toxicity) Test Results for WI Environmental

Tests show XR-88TM not harmful to fish or aquatic life

Stormwater samples were taken from Lake Union at CSR Marine in Seattle, Washington on June 11th, 2010. The samples were then treated with WI Environmental’s XR-88TM product. They were put into approved containers provided by Nautilus Environmental, packed into a cooler containing ice, and sent via FedEx overnight to Nautilus Environmental. Appropriate ‘chain of custody’ procedures were followed, according to Nautilus Environmental.

Nautilus Environmental is a Washington State accredited lab in Fife, meeting strict state guidelines and following internal quality control procedures. It is one of only a few commercial labs in the state to perform the WET test.

Upon arrival at Nautilus, the coolers were opened, samples inspected and the contents verified against information provided on the ‘chain of custody’ form. The temperature of the samples was measured and recorded, and samples were then stored at 4 degrees Celsius in the dark until used for testing.

Acute toxicity tests were carried out on June 11th, 2010 at 1pm, using water fleas (Daphnia pulex) and rainbow trout (Oncorhynchus mykiss) according to standard EPA and Washington State testing methods.

In the test using Daphnia pulex, the samples are diluted with clean laboratory water to concentrations at 100, 50, 25, 12.5 and 6.25 percent samples. At the end of 48 hours, 95% of the Daphnia pulex had survived in the 100% sample concentration, with no measurable difference seen at any of the various levels of concentrations compared to controls, showing XR-88TM is not harmful to aquatic invertebrates.

In the test using rainbow trout, the samples are again diluted with clean laboratory water to concentrations of 100, 50, 25, 12.6 and 6.25 percent samples. At the end of 96 hours, 100% of the rainbow trout had survived, with no measurable difference seen at any of the various levels of concentrations compared to controls, showing XR-88TM is not harmful to fish.